貨號
724211
12孔板+12小室，5 μm孔徑PC(聚碳酸酯)膜，半透明，未TC，滅菌
12 Well Plate+12 Inserts, 5 μm Pore Size PC(Polycarbonate) Membrane, Translucent, Non-TC, Sterile
產品訊息
細胞小室在細胞實驗中常用，如：共培養實驗、趨化實驗、細胞遷移實驗、細胞侵襲、藥物轉運。其中，通透性支持物可以有效改善極性細胞的培養，因為這些支持物允許細胞從其基底面和頂面分泌和吸收分子，從而以更為自然的方式進行代謝，最大程度的模擬體內環境以培養某些特殊的細胞系。
Cell and tissue culture technologies have an increasing importance in the fields of basic and applied life science. New culture vessels and new surfaces for cell adsorption are continuously emerging, in order to simulate the internal environment as much as possible for culture of some special cell lines. Logically, using permeable supports with a microporous membrane becomes the basic method for culturing these cells. Permeable supports may effectively improve the culture of polar cells, because these supports allow cells to secrete on and absorb molecules from their basal and apical surfaces to metabolize in a more natural way, as well as to stimulate the in vivo environment to the maximum extent for culturing of some special cell lines.
描述
產品詳情 Introduction
• 創新的邊緣設計，加樣方便
• PC膜：低吸附率，減少小分子蛋白和其他化合物的流失
• PET膜具有較好的光學清晰度，方便觀察細胞狀態
• 與大部分固定與染色時使用的溶劑相容
| 應用 | 細胞 | 膜孔徑 |
|---|---|---|
| ADME（化合物穿過腸上皮細胞屏障轉運和滲透） | Caco-2、MDCK | 0.4 μm |
| 共培養和細胞分化、細胞成像 | 原代細胞、腫瘤幹細胞 | 0.4 μm、1.0 μm |
| 大分子或病毒轉運、分泌 | 1.0 μm、3.0 μm | |
| 細胞遷移與侵襲（血管新生）、細胞趨化（遷移）或內皮遷移 軸突增生、共培養 | 內皮細胞、白血球 神經元 | 3.0 μm |
| 細胞遷移與侵襲 | 淋巴球、巨噬細胞 | 3.0 μm、5.0 μm |
| 腫瘤細胞遷移與侵襲 血球趨化作用或經內皮細胞遷移、共培養 | 腫瘤衍生細胞、白血球 腫瘤幹細胞、MSCs | 8.0 μm |
Introduction
• Sterilized by E-beam, SAL=10-6.
• Vacuum Plasma tissue culture treatment.
• Non-Pyrogenic, DNase/Rnase free.
• A rich selection of matching plates: 6-well, 12-well, 24-well.
• Passed the USP VI toxicity test.
• Cell culture plates are made of high clarity, 100% virgin polystyrene.
• Clear lot number for batch traceability.
• Innovative edge design for convenient sample loading.
• Low protein binding to ensure accurate results.
• Compatible with most solvents used for fixing and staining.
Attention：
• Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
•During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
•Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.
Guidelines for us：
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight. • Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Check the volume of the culture medium regularly and add fresh medium as needed.
• The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
